BAP1 Antilichaam / BRCA1-geassocieerd eiwit 1

The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Immunohistochemistry (FFPE) : 1-2 µg/mL

Q92560

Human

A portion of amino acids 400-729 from human BRCA1-Associated Protein 1 protein was used as the immunogen for the BAP1 antibody.

BAP1/9795R

IHC-P

Protein A affinity

Purified

This BAP1 antibody is available for research use only.

Aliquot the BAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the BAP1 antibody should be determined by the researcher.

Cytoplasm, Nucleus

IHC staining of FFPE human breast carcinoma tissue with BAP1 antibody (clone BAP1/9795R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.