Bax-antilichaam

Bcl-2 Associated X Protein is a member of the Bcl-2 gene family. The gene encodes a 21-kDa protein whose association with Bcl-2 is believed to play a critical role in regulating apoptosis. It is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases. It resides in an inactive state in the cytosol of many cells. Bax consists of 9 alpha helices and has extensive amino acid homology with Bcl-2, focused within highly conserved domains I and II. The protein is encoded by six exons and demonstrates a complex pattern of alternative RNA splicing that predicts a 21 kd membrane (alpha) and two forms of cytosolic protein (beta and gamma) . Bax and Bak are essential for regulating the number of B cells at both immature and mature developmental stages. They are critical mediators of B cell death induced by multiple stimuli.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q07812

Human, Mouse, Rat

Amino acids 19-33 (IMKTGALLLQGFIQD) were used as the immunogen for this Bax antibody.

WB, FACS, IHC-P

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

This Bax antibody is available for research use only.

After reconstitution, the Bax antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

The stated application concentrations are suggested starting points. Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western blot testing of Bax antibody and Lane 1: rat testis; 2: rat kidney; 3: (r) brain; 4: (r) ovary; 5: human HeLa; 6: (h) MM231; 7: (h) A549; 8: (h) Jurkat; 9: (h) placenta tissue lysate. Predicted molecular weight: 21-24 kDa.