BC-GLA-NHS

BC-GLA-NHS (CLIP-GLA-NHS) is a NHS-labeled CLIP tag, which is an amine-reactive crosslinker and can achieve site-specific protein labeling. The NHS ester of BC-GLA-NHS reacts with the primary amine of the target molecule (such as antibodies, quantum dots) in a pH 7.5-8.5 buffer solution to form a stable amide bond. After coupling, the BC molecule undergoes a nucleophilic substitution reaction with the active site (Cys) of the CLIP-tagged fusion protein, releasing benzylcytosine and forming an irreversible thioether bond, achieving covalent labeling. BC-GLA-NHS can be used for click chemistry labeling[1].

CLIP-GLA-NHS

12352005

Biochemical Assay Reagents

Others

Others

NC1=CC=NC(OCC2=CC=C(C=C2)CNC(CCCC(ON3C(CCC3=O)=O)=O)=O)=N1

C21H23N5O6

441.44

[1]Corrêa IR Jr. Considerations and protocols for the synthesis of custom protein labeling probes. Methods Mol Biol. 2015;1266:55-79.

Room temperature

http://file.medchemexpress.com/batch_PDF/HY-D2956/

Dye Reagents

No Development Reported