BCHE Antilichaam / Butyrylcholinesterase

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P06276

Human

E.coli-derived human BCHE recombinant protein (Position: E29-L602) was used as the immunogen for the BCHE antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This BCHE antibody is available for research use only.

After reconstitution, the BCHE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the BCHE antibody should be determined by the researcher.

Western blot analysis of BCHE using anti-BCHE antibody. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCHE antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A doublet is observed at ~85-90 kDa, higher than the predicted 68 kDa molecular weight. This shift is well documented and results from extensive N-linked glycosylation of the mature secreted enzyme, which adds ~15-20 kDa of carbohydrate mass. Variability in glycan composition or processing produces the characteristic doublet pattern seen for BCHE in multiple mammalian cell types.