BCKDH E2 Antilichaam / Dihydrolipoamide vertakte keten transacylase E2 / DBT

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P11182

Human, Mouse, Rat

E.coli-derived human DBT recombinant protein (Position: K46-R462) was used as the immunogen for the BCKDH E2 antibody.

WB, ICC/IF, IP, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This BCKDH E2 antibody is available for research use only.

After reconstitution, the BCKDH E2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the BCKDH E2 antibody should be determined by the researcher.

Mitochondria

Immunofluorescent staining of BCKDH E2/DBT using anti-BCKDH E2 antibody (green) and anti-Beta Tubulin antibody (red) . BCKDH E2/DBT was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-BCKDH E2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 550 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.