Bcl-2 Antilichaam

Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

596

P10415

AAH27258

Human, Mouse

3335

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain. Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3335 3337

Apoptosis, Autophagy, Cancer

Bcl-2 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1288 - A20 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)

Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Bcl-2antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD) . Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD) . Rat Bcl-2 has one isoform (236aa, 27kD) . 3337 can detect all isoforms of human, mouse and rat

BCL2

Cat. No. 1305 - Human Kidney Tissue Lysate

Cat. No. 10-401 - Human Kidney Tissue Slide

Cat. No. 1219 – MCF-7 Cell Lysate

Cat. No. 1208 – THP-1 Cell Lysate

B-cell CLL/lymphoma 2

Homo sapiens

Lockshin et al. Cell Death Differ. 2000; 7:2-7.

Cory et al. Oncogene 2003; 22:8590-607.

Heiser et al. Exp. Geron. 2004; 39:1125-35.

Hockenbery et al. Nature 1990; 348:334-6.

Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

ELISA, WB, IHC-P, IF

20072668

Liquid