BCL9L Antilichaam

B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903) . There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Western blot: 0.1-0.5 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

Q86UU0

Human, Mouse, Rat

Amino acids E397-A666 from the human protein were used as the immunogen for the BCL9L antibody.

WB, IHC-P, IF, Direct ELISA

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

This BCL9L antibody is available for research use only.

Store the BCL9L antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the BCL9L antibody should be determined by the researcher.

IHC staining of FFPE human esophagus squama cancer with BCL9L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.