BCMA Antilichaam [Vicky-1]

608

Q02223

Human

BCMA (monoclonal Vicky-1) antibody was raised against recombinant human BCMA:Fc.

Vicky-1

Unconjugated

Primary Antibodies

Chemokines & Cytokines, Cancer

BCMA (monoclonal Vicky-1) antibody was purified to 95% in SDS-PAGE.

Batch dependent

PBS containing 0.02% sodium azide

None

Blue Ice

BCMA antibody can be stored at 4˚ C, stable for one year.

IgG1

BCMA antibody can be used to recognize human BCMA by Flow Cytometry and immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5 μ g/mL Vicky-1 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of monoclonal antibody to BCMA (Vicky-1) . After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. This product is for research use only.

Tumor necrosis factor receptor superfamily, member 17

Homo sapiens

BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000) .

BAFF AND APRIL: a tutorial on B cell survival: F. Mackay, et al.; Annu. Rev. Immunol. 21, 231 (2003) .

ICC, Flow

Liquid