Bif-antilichaam

Bif Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.

51100

Q9Y371

AAK27365

Human, Mouse

Unconjugated

Primary Antibodies

Apoptosis, Innate Immunity

Bif Antibody is affinity chromatography purified via peptide column.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%), Bovine: (93%)

Bif Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Bif antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

SH3GLB1

Cat. No. 17-001 - HeLa Cell Slide

SH3-domain GRB2-like endophilin B1

Homo sapiens

Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.

Cuddeback SM, Yamaguchi H, Komatsu K, et al. Molecular cloning and characterization of bif-1. J. Biol. Chem.2001; 276:20559-65.

Takahashi Y, Karbowski M, Yamaguchi H, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol. Cell. Biol. 2005; 25:9369-82.

Bif Antibody: Bif-1, CGI-61, PPP1R70, dJ612B15.2, KIAA0491, Endophilin-B1, Bax-interacting factor 1, Bif-1

ELISA, WB, ICC

13469879

Liquid