Bik Antilichaam

Bik Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER) -residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) -only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.

638

Q13323

CAG30276

Human, Mouse

Unconjugated

Primary Antibodies

Apoptosis, Innate Immunity

Bik Antibody is affinity chromatography purified via peptide column.

Cat. No. 1205 - Jurkat Cell Lysate

1 mg/mL

Bik Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Bik antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

BIK

Cat. No. 17-005 - Jurkat Cell Slide

BCL2-interacting killer (apoptosis-inducing)

Homo sapiens

Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

Germain M, Mathai JP, and Shore GC. BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria. J. Biol. Chem. 277:18053-60.

Germain M, Mathai JP, McBride HM, et al. Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis. EMBO J. 2005; 24:1546-56.

Bik Antibody: BP4, NBK, BIP1, Bcl-2-interacting killer, Apoptosis inducer NBK

ELISA, WB, ICC, IF

47678311

Liquid