Biotinyleerd Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Proteïne, AvitagTM, His Tag

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

Biotinylated Influenza A virus (A/Victoria/4897/2022) NA (H1N1) Protein, Avitag, His Tag (NEE-V82Q3) is expressed from human 293 cells (HEK293) . It contains AA Val 80 - Asn 469 (Accession # EPI_ISL_17102775, GISAID) .

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Val 80 - Asn 469

Neuraminidase (NA)

Biotin-labeled

N-Avi & N-10xHis

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

90%

Powder

PBS, pH7.4

52.6 kDa

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RT

-20°C

25ug*1

HEK293

Influenza A virus (A/Victoria/4897/2022)

EPI_ISL_17102775

Trehalose