Bloedgroep Antigenen A Antilichaam / ABO

This mAb preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This mAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

P16442

Human

Mucin isolated from an ovarian cyst fluid was used as the immunogen for the Blood Group Antigen A antibody.

33C13

IHC-P

Protein G affinity chromatography

Purified

This Blood Group Antigen A antibody is available for research use only.

Store the Blood Group Antigen A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Blood Group Antigen A antibody should be determined by the researcher.

Cell surface

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen A antibody (33C13) . Staining of formalin-fixed tissues requires boiling tissue sections in pH6 10mM citrate buffer for 10-20 min followed by cooling at RT for 20 min.