Bloomsyndroom (fosfo Thr99) Antilichaam

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE) .

641

P54132

NP_000048.1

Human

Bloom Syndrome (phospho Thr99) antibody was raised against a peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p) -Q-R) derived from Human Bloom Syndrome.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

159 kDa

IgG

The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

BLM

Bloom syndrome, RecQ helicase-like

Homo sapiens

Ellis N.A., Cell 83:655-666 (1995) .

Karow J.K., J. Biol. Chem. 272:30611-30614 (1997) .

Pichierri P., EMBO J. 23:3154-3163 (2004) .

RECQ2, RECQL3, type 2, EC 3.6.1, RecQ protein-like 3

WB, IHC

1705486

Liquid