BMP 2

Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. The amino acid sequence of recombinant human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector. BMP-2 is a heparin binding protein.

Bone morphogenetic protein 2; BDA2; BMP2A; bone morphogenetic protein 4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6

650

P12643

NP_001191

NM_001200

20p12

Human/Murine/Rat

Human

MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

The lyophilized BMP-2 is best soluble in 50 mM acetic acid at a concentration of 0.1mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0.

< 0.1 ng per µg of BMP-2

> 95% by SDS-PAGE

Measured by the ability of BMP-2 to induce alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 µg/ml.

115

Lyophilized

50 mM acetic acid

50mM acetic acid

26.0 kDa

Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted BMP-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

E. coli

MQAKHKQ