BMP-7

TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling and maintenance of a variety of other tissues and organs. BMP-7, also known as osteogenic protein-1 or OP-1, is a potent bone inducing agent, which in the presence of appropriate osteoconductive carrier (e.g. collagen sponge or synthetic hydroxyapatite) can be used in the treatment of bone defects. A bone-graft substitute, called OP-1TM implant, made of recombinant human BMP-7 associated with bovine bone-derived collagen, has recently been approved by the FDA as a device for treating critical-size bone fractures. The potential use of BMP-7 in dental reconstructive surgeries is currently under investigation. Recombinant human BMP-7 is a homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 315 to 431 of the full-length BMP 7 precursor. Recombinant human BMP-7 has a calculated molecular weight of 26.4 kDa; however, due to glycosylation, the BMP-7 homodimer migrates at an apparent molecular weight of approx. 25-35 kDa by SDS-PAGE analysis under non-reducing conditions.

BMP7; OP-1

655

P18075

NP_001710.1

NM_001719.2

20q13

Human

Mouse, Human

MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH

Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at 20°C to -80°C

< 0.1 ng/µg of protein (< 1EU/µg)

98% by SDS-PAGE & HPLC analysis

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 μg/ml.

117

Lyophilized

PBS

Water

26.4 kDa

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 9 months at -20°C to -80°C.

CHO cells