BRCA1 (fosfo Ser1423) Antilichaam

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

672

P38398

NP_009225.1

Human

BRCA1 (Phospho-Ser1423) antibody was raised against a peptide sequence around phosphorylation site of serine 1423 (H-G-S (p) -Q-P) derived from Human BRCA1.

Unconjugated

Primary Antibodies

Phospho-Specific, Cell Cycle

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

220 kDa

This antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

BRCA1

Breast cancer 1, early onset

Homo sapiens

Kim ST, et al. (2002) Genes Dev; 16 (5) : 560-570Arlt MF, et al. (2004) Mol Cell Biol; 24 (15) : 6701-6709Zhang J, et al. (2004) Mol Cell Biol; 24 (2) : 708-718Greenberg RA, et al. (2006) Genes Dev; 20 (1) : 34-46Tibbetts RS, et al. (2000) Genes Dev; 14 (23) : 2989-3002

IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53, ERYF1, GF1

WB, IHC

6552299

Liquid