BRCA2 (fosfo Ser3291) Antilichaam

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA) . Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.

675

P51587

NP_000050.2

Human, Mouse, Rat

BRCA2 (phospho Ser3291) antibody was raised against a peptide sequence around phosphorylation site of Serine 3291 (F-V-S (p) -P-A) derived from Human BRCA2.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

>350 kDa

IgG

The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291.

Western Blot: 1:500~1:1000

BRCA2

Breast cancer 2, early onset

Homo sapiens

Wooster R., Nature 378:789-792 (1995) .

Tavtigian S.V., Nat. Genet. 12:333-337 (1996) .

Dunham A., Nature 428:522-528 (2004) .

BRCC2, BRCA2, subunit 2, BRCA1/BRCA2-containing complex

WB

14424438

Liquid