BspEI

BspEI is a Type IIP restriction enzyme derived from Bacillus and obtained by recombinant expression and purification in Escherichia coli. BspEI recognizes and cleaves the sequence TCCGGA, generating a 4-base 5' overhang. BspEI requires the dedicated Cut Buffer C and is incompatible with the universal CutOne® Buffer.

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG25528.pdf

• BspEI (10 U/μl) : 50 μl • 10x Cut Buffer C: 1 ml

Recommended Reaction Conditions 1x Cut Buffer C; Incubate at 37°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dam methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Store at -20°C for 2 years.

Kpn2I, MroI, AccIII, Aor13HI, Bsp13I, BseAI

TCCGGA 5'...T↓C C G G A...3' 3'...A G G C C↑T...5'