BTF3 cDNA

BTF3 encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.

BETA-NAC, BTF3a, BTF3b, NACB

5q13.2

Human

PATGen (puc19-derived cloning vector)

ATGCGACGGACAGGCGCACCCGCTCAGGCTGACTCTCGGGGGCGAGGTCGAGCCAGGGGCGGCTGCCCTGGGGGCGAGGCGACGCTGTCTCAACCTCCACCTCGCGGCGGAACCCGAGGACAGGAGCCTCAGATGAAAGAAACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCACAAGTGCGCATTGGTGGGAAAGGAACTGCTCGCAGAAAGAAGAAGGTGGTTCATAGAACAGCCACAGCAGATGACAAAAAACTTCAGTTCTCCTTAAAGAAGTTAGGGGTAAACAATATCTCTGGTATTGAAGAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCACTTTAACAACCCTAAAGTTCAGGCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACAAAGCAGCTGACAGAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACTAGTTTAAGGAGACTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTTGCTACTGGAGAGGATGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCTTCCAAGAATGAGGCAAACTGA

BTF3, BETA-NAC, BTF3a, BTF3b, NACB, BTF3, ATGD0330-10 µg, ATGD0330-20 µg, ATGD0330-50 µg, ATGD0330-100 µg, ATGD0330-250 µg, ATGD0330-500 µg, ATGD0330-1 mg, ATGD0330-10, ATGD0330-20, ATGD0330-50, ATGD0330-100, ATGD0330-250, ATGD0330-500, ATGD0330-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

602542

NP_001032726.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001037637.1

621bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.