Bub3 Antilichaam

Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

9184

O43684

O43684

Human, Mouse

Unconjugated

Primary Antibodies

Homeostasis, Signal Transduction

Bub3 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1205 - Jurkat Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Bub3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

BUB3

Cat. No. 17-005 - Jurkat Cell Slide

Budding uninhibited by benzimidazoles 3 homolog (yeast)

Homo sapiens

Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev.2000; 14:2277-82.

Taylor SS, Ha E and McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol.1998; 142:1-11.

Warren CD, Brady DM, Johnston RC, et al. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol. Biol. Cell2002; 13: 3029-41.

Roberts BT, Farr KA and Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol.1994; 14:8282-91.

Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint protein BUB3

ELISA, WB, ICC, IF

7387554

Liquid