C-RAF (fosfo Ser642) Antilichaam

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) . The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1) . Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

5894

P04049

NP_002871.1

Human, Mouse, Rat

C-RAF (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (T-T-S (p) -P-R) derived from Human C-RAF.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

74 kDa

IgG

The antibody detects endogenous levels of C-RAF only when phosphorylated at serine 642.

Western Blot: 1:500~1:1000

RAF1

V-raf-1 murine leukemia viral oncogene homolog 1

Homo sapiens

Bonner T.I., Nucleic Acids Res. 14:1009-1015 (1986) .

Ota T., Nat. Genet. 36:40-45 (2004) .

Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

C-RAF, C-Raf, CRAF, RAF-1

WB

4506401

Liquid