C1QA Antibody / Complement component 1 subcomponent subeenheid A

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

P02745

Human, Rat

A synthesized peptide derived from human C1QA was used as the immunogen for the C1QA antibody.

31C57

WB, IHC

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This C1QA antibody is available for research use only.

Store the C1QA antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the C1QA antibody should be determined by the researcher.

Western blot analysis of C1QA using anti-C1QA antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HCCT whole cell lysates, Lane 2: human HCCP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QA antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for C1QA at approximately 28 kDa. The expected molecular weight of C1QA is ~28 kDa.