C3H/1012 Lysaat

C3H/10T1/2 cell line was isolated in 1972 from a line of C3H mouse embryo cells. The cells are contact sensitive and there is no detectable background spontaneous transformation. They are highly susceptible to transformation by chemical agents and express H-2k antigen. Tested and found negative for ectromelia virus.

Mouse

WB

2 mg/mL (determined by BCA assay)

C3H01, ATGL0022-10 µg, ATGL0022-20 µg, ATGL0022-50 µg, ATGL0022-100 µg, ATGL0022-250 µg, ATGL0022-500 µg, ATGL0022-1 mg, ATGL0022-10, ATGL0022-20, ATGL0022-50, ATGL0022-100, ATGL0022-250, ATGL0022-500, ATGL0022-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : C3H/101/2 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody