Cadherine-2 Antilichaam / N-Cadherine / CDH2

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P19022

Human

A recombinant human Cadherin 2 partial protein from the cytoplasmic domain was used as the immunogen for the Cadherin 2 antibody.

CDH2/9491

IHC-P

Protein G affinity

Purified

This Cadherin-2 antibody is available for research use only.

Aliquot the Cadherin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Cadherin 2 antibody should be determined by the researcher.

Cell Surface, Cytoplasm

IHC staining of FFPE human heart tissue with Cadherin 2 antibody (clone CDH2/9491) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.