CaMK2 alfa/beta/delta (fosfo Thr305) Antilichaam

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

816;817

Q9UQM7

NP_741960.1

Human, Mouse, Rat

CaMK2 alpha/beta/delta (phospho Thr305) antibody was raised against a peptide sequence around phosphorylation site of threonine 305 (I-L-T (p) -T-M) derived from Human CaMK2alpha/beta/delta.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

54 kDa

IgG

The antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 305.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

CAMK2B, CAMK2D

Calcium/calmodulin-dependent protein kinase II beta, calcium/calmodulin-dependent protein kinase II delta

Homo sapiens

Carl W. Tong, J. Physiol., Aug 2004; 558: 927 - 941.

Pierre R, J. Biol. Chem., Sep 1997; 272: 24133.

Daliang Wang, PNAS, Jun 1998; 95: 7133.

CAMK2A, KCC2A, kinase CaMK2-alpha, CaMKII-alpha

WB, IHC

25952118

Liquid