CASP8 Antilichaam / Caspase 8 (kleine subeenheid)

Caspase 8 is also known as CAP4, MACH or MCH5. The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells

841

Q14790

Human, Mouse, Rat

Amino acids 410-449 (VSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYE-human) were used as the immunogen for the CASP8 antibody.

WB, IHC-P, FACS

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

This CASP8 antibody is available for research use only.

After reconstitution, the CASP8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the CASP8 antibody should be determined by the researcher.

Cytoplasmic

IHC testing of FFPE human breast cancer tissue with CASP8 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.