Caspase-9 Antilichaam

Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα , TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

842

P55211

P55211

Human, Mouse, Rat

2071

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 1) shows similar Caspase 9 expression profile in human cell lines detected by two independent anti-Caspase 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain. Caspase 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2071 2073

Apoptosis, Cancer

Caspase-9 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/ml

Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Caspase-9 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

It has no cross response to other members in caspase family.

Human Caspase 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD) . Mouse Caspase 9 has one isoform (454aa, 50kD) and rat Caspase 9 also has one isoform (454aa, 50kD) . 2071 can detect human, mouse and rat.

CASP9

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1466 - Rat Spleen Lysate

Caspase 9, apoptosis-related cysteine peptidase

Homo sapiens

Duan et al. J Biol Chem 1996;271:16720-4.

Srinivasula et al. J Biol Chem 1996;271:27099-106.

Li et al. Cell 1997;91:479-89.

Pan et al. J Biol Chem 1998;273:5841-5 (RD1299) .

Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9

ELISA, WB

28558771

Liquid