Caspase8 Antilichaam

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

58022

Q14790

NP_571585.2

Human

Caspase8 antibody was raised against a peptide sequence around aa.221~225 (T-L-D-K-V) derived from Human Caspase8.

Unconjugated

Primary Antibodies

Apoptosis, Cancer , Inflammasomes

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

None

Blue Ice

Store antibody at -20˚ C for up to one year.

18, 43, 57 kDa

This antibody detects endogenous level of total Caspase8 protein.

Western Blot: 1:500~1:1000

Casp8

Caspase 8, apoptosis-related cysteine peptidase

Homo sapiens

Himeji D. et. al. (2002) Blood 99:4070-4078Breckenridge D. G. et. al. (2002) Proc. Natl. Acad. Sci. U. S. A. 99:4331-4336Muzio M. et. al. (1997) J. Biol. Chem. 272:2952-2956

Caspase-8, zgc:92075, NCAM, CASP8, MCH5

WB

52138561

Liquid