CBFB cDNA

CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e. g., RuNX1) and osteogenesis (e. g., RuNX2) . This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit.

PEBP2B

16q22.1

Human

PATGen (puc19-derived cloning vector)

ATGCCGCGCG TCGTGCCCGA CCAGAGAAGC AAGTTCGAGA ACGAGGAGTT TTTTAGGAAG CTGAGCCGCG AGTGTGAGAT TAAGTACACG GGCTTCAGGG ACCGGCCCCA CGAGGAACGC CAGGCACGCT TCCAGAACGC CTGCCGCGAC GGCCGCTCGG AAATCGCTTT TGTGGCCACA GGAACCAATC TGTCTCTCCA GTTTTTTCCG GCCAGCTGGC AGGGAGAACA GCGACAAACA CCTAGCCGAG AGTATGTCGA CTTAGAAAGA GAAGCAGGCA AGGTATATTT GAAGGCTCCC ATGATTCTGA ATGGAGTCTG TGTTATCTGG AAAGGCTGGA TTGATCTCCA AAGACTGGAT GGTATGGGCT GTCTGGAGTT TGATGAGGAG CGAGCCCAGC AGGAGGATGC ATTAGCACAA CAGGCCTTTG AAGAGGCTCG GAGAAGGACA CGCGAATTTG AAGATAGAGA CAGGTCTCAT CGGGAGGAAA TGGAGGTGAG AGTTTCACAG CTGCTGGCAG TAACTGGCAA GAAGACAACA AGACCCTAG

CBFB, PEBP2B, CBFB, ATGD0021-10 µg, ATGD0021-20 µg, ATGD0021-50 µg, ATGD0021-100 µg, ATGD0021-250 µg, ATGD0021-500 µg, ATGD0021-1 mg, ATGD0021-10, ATGD0021-20, ATGD0021-50, ATGD0021-100, ATGD0021-250, ATGD0021-500, ATGD0021-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

121360

NP_001746.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001755.2

549bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.