CCR1 Antilichaam / C-C chemokine receptor type 1

Western Blot: 0.25-0.5 µg/mL

P51675

Mouse, Rat

A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCR1 was used as the immunogen for the CCR1 antibody.

WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This CCR1 antibody is available for research use only.

After reconstitution, the CCR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the CCR1 antibody should be determined by the researcher.

Western blot analysis of CCR1 using anti-CCR1 antibody. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for CCR1 at approximatel 41 kDa. The expected molecular weight of CCR1 is ~41 kDa.