CCS cDNA

CCS is essential for the incorporation of copper into SOD-1, and therefore is necessary for its enzymatic activity. CCS prevents copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the necessary copper cofactor. CCS escorts copper only to SOD-1 and fails to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. While many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neuropil of the central nervous system.

11q13

Human

PATGen (puc19-derived cloning vector)

ATGGCTTCGG ATTCGGGGAA CCAGGGGACC CTCTGCACGT TGGAGTTCGC GGTGCAGATG ACCTGTCAGA GCTGTGTGGA CGCGGTGCGC AAATCCCTGC AAGGGGTGGC AGGTGTCCAG GATGTGGAGG TGCACTTGGA GGACCAGATG GTCTTGGTAC ACACCACTCT ACCCAGCCAG GAGGTGCAGG CTCTCCTGGA AGGCACGGGG CGGCAGGCGG TACTCAAGGG CATGGGCAGC GGCCAGTTGC AGAATCTGGG GGCAGCAGTG GCCATCCTGG GGGGGCCTGG CACCGTGCAG GGGGTGGTGC GCTTCCTACA GCTGACCCCT GAGCGCTGCC TCATCGAGGG AACTATTGAC GGCCTGGAGC CTGGGCTGCA TGGACTCCAC GTCCATCAGT ACGGGGACCT TACAAACAAC TGCAACAGCT GTGGGAATCA CTTTAACCCT GATGGAGCAT CTCATGGGGG CCCCCAGGAC TCTGACCGGC ACCGCGGAGA CCTGGGCAAT GTCCGTGCTG ATGCTGACGG CCGCGCCATC TTCAGAATGG AGGATGAGCA GCTGAAGGTG TGGGATGTGA TTGGCCGCAG CCTGATTATT GATGAGGGAG AAGATGACCT GGGCCGGGGA GGCCATCCCT TATCCAAGAT CACAGGGAAC TCCGGGGAGA GGTTGGCCTG TGGCATCATT GCACGCTCCG CTGGCCTTTT CCAGAACCCC AAGCAGATCT GCTCTTGCGA TGGCCTCACC ATCTGGGAGG AGCGAGGCCG GCCCATCGCT GGCAAGGGCC GAAAGGAGTC AGCGCAGCCC CCTGCCCACC TTTGA

CCS, CCS, ATGD0069-10 µg, ATGD0069-20 µg, ATGD0069-50 µg, ATGD0069-100 µg, ATGD0069-250 µg, ATGD0069-500 µg, ATGD0069-1 mg, ATGD0069-10, ATGD0069-20, ATGD0069-50, ATGD0069-100, ATGD0069-250, ATGD0069-500, ATGD0069-1

Store the plasmid at -20C.

Lyophilized

Cancer

603864

NP_005116.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_005125.1

825bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.