CD130 Antilichaam / IL6ST / Interleukin 6 Receptor subunit beta

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM) . This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17.

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25

P40189

Human

A portion of amino acids 871-899 from the human protein were used as the immunogen for the CD130 antibody.

WB, IHC-P

Antigen affinity purified

Purified

This CD130 antibody is available for research use only.

Aliquot the CD130 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the CD130 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western blot testing of human 1) HeLa, 2) MDA-MB-231, 3) HepG2, 4) SW480 and 5) A549 cell lysate with CD130 antibody. Expected molecular weight: 104-140 kDa depending on glycosylation level.