CD142 (Tissue factor)

Tissue factor is a transmembrane glycoprotein of the cytokine receptor superfamily that acts as a receptor for coagulation factor VII (fVII) to trigger initiation of the coagulation cascade in response to vascular injury. Expression of tissue factor occurs constitutively within most extravascular and perivascular cells and at high levels within critical organs and tissue. Tissue factor is not normally expressed freely on the surface of circulating blood cells due to its pro‐coagulant effect, but is instead stored on the surface of mononuclear and endothelial cells in microparticles that can shed into circulation in response to vascular injury, pro‐inflammatory cytokines, or microbial ligands. Tissue factor can also be shed into circulation by cancer cells where its expression in a number of cancer types has been linked to tumor progression, metastatic potential, thrombosis, and angiogenesis. Expression of tissue factor has been shown to be inducible by select cytokines in a number of cell types, including IL‐1β and TNF‐α in vascular endothelial cells and macrophages, and TNF‐α, IL‐6, and FGF‐Basic in monocytes, among others. The CHO cell-derived Recombinant Human Tissue Factor is a 227-amino-acid length glycoprotein containing a C-terminal His-Tag and has a calculated molecular weight of 25.8 kDa. Due to glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 30-40 kDa by SDS-PAGE gel under reducing and non-reducing conditions.

TF, TF1, Coagulation factor III, factor III, F3, Thromboplastin, CD142

2152

P13726

NP_001984.1

NM_001993.4

Human

Human

His-Tag, C-term

SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GGEKGEFREH HHHHHHH

< 0.1 ng/µg of protein (< 1EU/µg)

≥ 98% by SDS-PAGE gel and HPLC analyses.

Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII.

227

Lyophilized

25.8 kDa

CHO cells