CD274 Proteïne (C-His-AVI, Cynomolgus, Rhesus)

CD274

C-His-AVI

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

1.Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (His & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Cynomolgus PD-1 Protein (hFc Tag) (Cat: 90311-C02H), the EC50 is 1.5-4.5 ng/mL (Routinely tested) . 2.Recombinant Cynomolgus PD-1 Protein, hFc Tag (Cat. No. 90311-C02H) captured on Protein A chip, can bind Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, His & AVI Tag (Cat. No. 90251-C49H-B) with an affinity constant of 2.890 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (His & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Anti-PDL1 (Research Grade Atezolizumab Biosimilar), the EC50 is 2.0-6.5 ng/mL (Routinely tested) . 4.Immobilized Anti-PDL1 (Research Grade Atezolizu*mab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (His & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C49H-B) the EC50 is 2.6 ng/mL (Routinely tested) .

Met1-Thr239

The recombinant Cynomolgus / Rhesus PD-L1 consists of 247 amino acids and predicts a molecular mass of 28.55 kDa. It migrates as an approximately 38.6 kDa band in SDS-PAGE under reducing conditions.

Cynomolgus, Rhesus