CD274 Proteïne (C-His, Human)

CD274

B7-H, B7-H1, B7H1, hPD-L1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

C-His

< 1.0 EU per μg protein as determined by the LAL method.

> 95% as determined by SDS-PAGE.

1.Immobilized Anti-PDL1 Antibody, Human IgG1 (Atezolizumab) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag), Biotinylated (Cat: 10084-H08H-B), the EC50 is 0.5-1.5 ng/mL (QC tested) . 2.Loaded Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) on proA Biosensor, can bind Biotinylated Recombinant Human PD-L1/B7-H1 Protein, His Tag (Cat. No. 10084-H08H-B) with an affinity constant of 0.873 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met1-Thr239

The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the human B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.

Human