CD28 Antibody37.51]

The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer. It is expressed on most thymocytes, at low density on most of the CD4+ and CD8+ peripheral T lymphocytes, and on the natural killer cells. It is additionally found on activated splenocytes, mast cells, and thymocytes. CD 28 is a costimulator of T lymphocytes and a ligand for CD80 (B7-1), and CD86 (B7-2) . The CD28 signaling stimulates the expression of IL-2 and IL-2 receptor and the cytotoxicity of CD3-activated T lymphocytes.The 37.51 antibody stimulates the proliferation of T lymphocytes and natural killer cells and their respective cytokine production.

12487

P31041

Mouse

37.51

Unconjugated

Primary Antibodies, Functional Antibodies

Immunology

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Batch dependent

Phosphate-buffered aqueous solution, ph7.2.

None

Blue Ice

The product should be stored undiluted at 4˚ C . Do not freeze.

Golden Syrian Hamster IgG

The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer.

Cd28

CD28 antigen

Mus musculus

Nandi, D., Gross, J. A., Allison, J. P. (1994) . CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells.The Journal of Immunology,152 (7), 3361-3369.

Gross, J. A., Callas, E. L. E. N. I., Allison, J. P. (1992) . Identification and distribution of the costimulatory receptor CD28 in the mouse.The Journal of Immunology,149 (2), 380-388.

Grossi, J. A., Raulet, D. H., Allison, J. P. (1992) . CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.Nature,356, 607-9.

Nishio, M., Spielman, J., Lee, R. K., Nelson, D. L., Podack, E. R. (1996) . CD80 (B7. 1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis.The Journal of Immunology,157 (10), 4347-4353.

CH29-189K17.3, Cd28

Flow, Functional Assays, IP

Liquid