CD28-eiwit (C-human IgG1-Fc, Cynomolgus)

CD28

C-human IgG1-Fc

< 1.0 EU per μg protein as determined by the LAL method.

> 90 % as determined by SDS-PAGE.

1. Immobilized Recombinant Human CD86 / B7-2 Protein (His Tag) (Cat:10699-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD) (Cat:90182-C02H), the EC50 is 100-420 ng/mL. 2. Immobilized Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat:10698-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD) (Cat:90182-C02H), the EC50 is 600-1400 ng/mL. 3.Loaded Recombinant Cynomolgus CD28 Protein, hFc Tag (Cat. No. 90182-C02H) on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein, His Tag (Cat. No. 10698-H08H) with an affinity constant of 0.126 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Cynomolgus CD28 Protein, hFc Tag (Cat. No. 90182-C02H) on proA Biosensor, can bind Recombinant Human B7-2/CD86 Protein, His Tag (Cat. No. 10699-H08H) with an affinity constant of 0.359 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met1-Pro152

The recombinant cynomolgus CD28 consists 372 amino acids and predicts a molecular mass of 41.9 kDa.

Cynomolgus