CD31 / PECAM1 eiwit (Fc-tag)

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

CD31 Protein; CD31/EndoCAM Protein; endoCAM Protein; GPIIA' Protein; PECA1 Protein; PECAM-1 Protein

EAW94208.1

Human

Human

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

> 90 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 10E4 cells/well are added to sCD31-Fc coated plates (5 μg/mL and 100 μL/well) in the presence of 10 μg/ml PHA approximately >60% will adhere after 30 minutes at 37°C.

812

Lyophilized

100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

See Datasheet

130 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells