CD355 Antilichaam [MTA-1]

The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

21936

O35714

Mouse

DTA-1

Unconjugated

Primary Antibodies, Functional Antibodies

Immunology

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Batch dependent

Phosphate-buffered aqueous solution, ph7.2.

None

Blue Ice

The product should be stored undiluted at 4˚ C . Do not freeze.

Rat IgG2b

The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.

Tnfrsf18

Tumor necrosis factor receptor superfamily, member 18

Mus musculus

Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... Sakaguchi, S. (2005) . Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells.The Journal of experimental medicine,202 (7), 885-891.

Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., Sakaguchi, S. (2002) . Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance.Nature immunology,3 (2), 135-142.

Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., Waldmann, H. (2003) . Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.Proceedings of the National Academy of Sciences,100 (25), 15059-15064.

AITR, Gitr, Tnfrsf18

Flow, Functional Assays, IHC, IF

Liquid