CD3D & CD3E eiwit (C-Vlag & C-His, menselijk)

CD3D & CD3E

C-Flag & C-His

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein, Biotinylated (Cat: CT038-H2508H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 antibody, Mouse IgG2a (Muromonab), the EC50 is 1-3 ng/mL. 2.Amine-Labeled Biotinylated Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat. No. CT038-H2508H-B) captured on CM5 chip via Anti-His antibody, can bind OKT3 derived Anti-CD3 antibody, Mouse IgG2a with an affinity constant of 0.241 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Met1-Ala105 & Met1-Asp126

The recombinant human CD3D&CD3E consists of 271 (126+145) amino acids and predicts a molecular mass of 31.05 (14.4+16.65) KDa.

Human