CD4-eiwit (C-His, mens)

CD4

CD4mut, IMD79, Leu-3, OKT4D, T4

C-His

< 1.0 EU per μg protein as determined by the LAL method.

1.Flow cytometric analysis of anti-CD4 CAR expression. 5e5 of anti-CD4 CAR-293 cells were stained with 100 μL of 1:100 dilution (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human CD4 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10400-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated CD4 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD4 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10400-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

Met1-Trp390

The protein has a predicted molecular mass of 44 kDa.

Human