CD45 Antilichaam [30-F11] (Violet-450)

The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA) . CD45 is a transmembrane glycoprotein, expressed by all the hematopoietic cells, except for platelets and mature erythrocytes, which distinguishes the leukocytes from the non-hematopoietic cells. The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons.The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

19264

P06800

Mouse

30-F11

Violet-450

Primary Antibodies

Immunology

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Batch dependent

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

None

Blue Ice

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Rat IgG2b, kappa

The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA) .

Ptprc

Protein tyrosine phosphatase, receptor type, C

Mus musculus

Ledbetter, J. A., Herzenberg, L. A. (1979) . Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*.Immunological reviews,47 (1), 63-90.

Thomas, M. L. (1989) . The leukocyte common antigen family.Annual review of immunology,7 (1), 339-369.

Simon, D. I., Chen, Z., Seifert, P., Edelman, E. R., Ballantyne, C. M., Rogers, C. (2000) . Decreased neointimal formation in Mac-1/mice reveals a role for inflammation in vascular repair after angioplasty.Journal of Clinical Investigation,105 (3), 293-300.

Loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4, Ptprc

Flow

Liquid