CD55 Recombinant Antilichaam [LU30] (OAAV00325)

CD8 antigen, beta chain 1

Cd8b; Ly-; Ly-3; Ly-C; lymphocyte antigen 3; Lyt-; Lyt-3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3.

12526

P26011

NP_033988.1

Mouse

Mouse CD8 (lyt-3) .

YTS 156.7.7

Recombinant Monoclonal Antibody

Purified

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs) . In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs) . Additionally, plays a critical role in thymic selection of CD8+ T-cells.

PBS with 0.02% Proclin 300.

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

IgG2b, kappa

The antibody recognises the B epitope of the mouse Lyt-3 (CD8) antigen. Has been used in CD8 subset depletion in vivo and flow cytometry studies.

Cd8b1

Rat

T-cell surface glycoprotein CD8 beta chain

Cd8b1

NM_009858.2