CD69 Antilichaam

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q07108

Human

E.coli-derived human CD69 recombinant protein (Position: M1-K199) was used as the immunogen for the CD69 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This CD69 antibody is available for research use only.

After reconstitution, the CD69 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the CD69 antibody should be determined by the researcher.

Western blot analysis of CD69 using anti-CD69 antibody. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD69 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for CD69 is at 23 kDa but may be observed at higher molecular weights due to glycosylation.