CD74 Antilichaam / CLIP

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P04233

Human

A recombinant partial protein from human CD74 protein was used as the immunogen for the CD74 antibody.

CLIP/7947

IHC-P, WB

Protein G affinity

Purified

This CD74 antibody is available for research use only.

Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the CD74 antibody should be determined by the researcher.

Cell membrane

IHC staining of FFPE human tonsil tissue with CD74 antibody (clone CLIP/7947) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.