CD8a Antilichaam [53-6,7]

The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha’ chain, of the mouse CD8 antigen. The alpha’ chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha’ chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b.CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha’-like polypeptides on some thymic and peripheral lymphocytes.

12525

P01731

Mouse

53-6.7

Unconjugated

Primary Antibodies, Functional Antibodies

Immunology

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Batch dependent

Phosphate-buffered aqueous solution, ph7.2.

None

Blue Ice

The product should be stored undiluted at 4˚ C . Do not freeze.

Rat IgG2a, kappa

The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha’ chain, of the mouse CD8 antigen.

Cd8a

CD8 antigen, alpha chain

Mus musculus

Ledbetter, J. A., Herzenberg, L. A. (1979) . Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*.Immunological reviews,47 (1), 63-90.

Ledbetter, J. A., Rouse, R. V., Micklem, H. S., Herzenberg, L. A. (1980) . T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.The Journal of experimental medicine,152 (2), 280-295.

Hathcock, K. (1991) . T cell enrichment by cytotoxic elimination of B cells and accessory cells.Current protocols in immunology, 3-3.

Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, Cd8a

Flow, Functional Assays, IHC, IP

Liquid