CD99 Antibody Cocktail

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32) . Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

P14209

Human

Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) were used as the immunogen for the CD99 antibody cocktail.

12E7 + MIC2/877

IHC-P

Unpurified high titer supernatant

Bioreactor concentrate

This CD99 antibody cocktail is available for research use only.

Store the CD99 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Cell surface

IHC: Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 antibody (clones 12E7 + MIC2/877) . Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.