Cdc25C (fosfo Ser216) Antilichaam

Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

995

P30307

NP_001781.2

Human

Cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C.

Unconjugated

Primary Antibodies

Phospho-Specific, Cell Cycle

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

60 kDa

This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

CDC25C

Cell division cycle 25C

Homo sapiens

Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4) : 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7) : 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7) : 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4) : 1990-1995.

CDC25, PPP1R60, MACS, PRKCSL, CDC25M1, MPIP3

WB, IHC, IF

125625350

Liquid