CDC34 cDNA

CDC34 is a member of the ubiquitin-conjugating enzyme family. ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. CDC34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae CDC34, which is essential for the G1 to S phase transition.

Cell division cycle 34, Ubiqiutin conjugating enzyme, Ubiquitin-conjugating enzyme E2 R1, E2 ubiquitin-conjugating enzyme R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, E2-CDC34, UBE2R1, UBC3, UBCH3

19p13.3

Human

PATGen (puc19-derived cloning vector)

ATGGCTCGGC CGCTAGTGCC CAGCTCGCAG AAGGCGCTGC TGCTGGAGCT CAAGGGGCTG CAGGAAGAGC CGGTCGAGGG ATTCCGCGTG ACACTGGTGG ACGAGGGCGA TCTATACAAC TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTACG AGGGCGGCTA CTTCAAGGCG CGCCTCAAGT TCCCCATCGA CTACCCATAC TCTCCACCAG CCTTTCGGTT CCTGACCAAG ATGTGGCACC CTAACATCTA CGAGACGGGG GACGTGTGTA TCTCCATCCT CCACCCGCCG GTGGACGACC CCCAGAGCGG GGAGCTGCCC TCAGAGAGGT GGAACCCCAC GCAGAACGTC AGGACCATTC TCCTGAGTGT GATCTCCCTC CTGAACGAGC CCAACACCTT CTCGCCCGCA AACGTGGACG CCTCCGTGAT GTACAGGAAG TGGAAAGAGA GCAAGGGGAA GGATCGGGAG TACACAGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGTGACGGC GTGAAGGTGC CCACCACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC GAGGGCTCAG ACCTCTTCTA CGACGACTAC TACGAGGACG GCGAGGTGGA GGAGGAGGCC GACAGCTGCT TCGGGGACGA TGAGGATGAC TCTGGCACGG AGGAGTCCTG A

CDC34, E2-CDC34, UBC3, UBCH3, UBE2R1, CDC34, ATGD0073-10 µg, ATGD0073-20 µg, ATGD0073-50 µg, ATGD0073-100 µg, ATGD0073-250 µg, ATGD0073-500 µg, ATGD0073-1 mg, ATGD0073-10, ATGD0073-20, ATGD0073-50, ATGD0073-100, ATGD0073-250, ATGD0073-500, ATGD0073-1

Store the plasmid at -20C.

Lyophilized

Enzymes & Proteases

116948

NP_004350.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004359.1

711bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.