CDH2 Antilichaam / Cadherine 2

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

P19022

Human

A portion of amino acids 400-600 from human Cadherin 2 protein was used as the immunogen for the CDH2 antibody.

CDH2/9492

IHC-P, WB

Protein G affinity

Purified

This CDH2 antibody is available for research use only.

Aliquot the CDH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the CDH2 antibody should be determined by the researcher.

Cell surface, cytoplasm

IHC staining of FFPE human heart tissue with CDH2 antibody (clone CDH2/9492) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.